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主题 : pls help me! esp. 清水MM
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楼主  发表于: 2011-03-16   

pls help me! esp. 清水MM

管理提醒: 本帖被 伍胥之 执行取消加亮操作(2011-03-16)
宽叶独行菜(Lepidium latifolium)是十字花科一种耐盐能力超过盐芥而且对低温、旱涝等多种逆境条件具有广谱耐性的植物,广布于我国及世界各地。盐芥的耐盐机理已经有许多研究,目前认为它的耐逆性与多种胁迫相关基因的组成型表达有关,它的根系钠钾离子选择性高于拟南芥。宽叶独行菜的耐逆性与盐芥有一定相似性,但耐逆范围与耐逆能力均远高于盐芥。然而,宽叶独行菜耐逆的分子机理研究仍然处于空白。宽叶独行菜与盐芥这类广谱耐逆植物是否具有类似的分子机理?这种机理在没有形态解剖上特化结构的植物中是否具有普遍意义?这些问题都具有十分重要的科学意义。本课题以宽叶独行菜为材料,在已有的耐盐生理研究基础上,以拟南芥和盐芥的基因组与转录组为参考,对宽叶独行菜进行比较基因组和转录组研究,并克隆部分耐逆相关基因,研究其表达调控模式,为将来利用该植物进行十字花科作物的耐逆性遗传改良提供参考。
[ 此帖被伍胥之在03-16-2011 19:12重新编辑 ]
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杺栫杣杊椌柮栬,䒴蓉艿芖。
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沙发  发表于: 2011-03-16   
Lepidium latifolium is a salt tolerant and resistant to many stress environment, which belong to Brassicaceae and is a relative to both Arabidopsis and Thellungiella halophila. In northern America, it is classified as a flooding-tolerant and invasive species, but in China, it is widely distributed in salt, drought and saline soils. In common sense, the molecular mechanism of T. halophila to salt and other stress tolerance is closely related to constitutive(组成型) expession of a series of REDOX genes, such as SOD, POD,and P5CS etc. their roots can descriminate Na+ and K+ more efficiently than that of Arabidopsis. The profile of stress of L. latifolium is very similar to T. halophila. Yet, the molecular mechanism of L. latifolium is still very spare(这里该用什么?). Is there any common pathway/mechanism in these plants which do not have mophological and structural spcieation/differentiation? It is of significance/importance in science in unversal stress tolerance. This grant/proposal  will use L. latifolium as material and study the comparative transcrptome, genome, based on the genome and transcriptome of Arabidopsis and Thellungiella. It will aim at clone some stress tolerant genes. This may help in genetic improvement of salt and other stress tolerance of crops in Brassicaeae.
[ 此帖被伍胥之在03-16-2011 09:31重新编辑 ]
杺栫杣杊椌柮栬,䒴蓉艿芖。
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板凳  发表于: 2011-03-16   
有人可以在8hr内帮我改一下吗? \\13n4fAv  
杺栫杣杊椌柮栬,䒴蓉艿芖。
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地板  发表于: 2011-03-16   
伍教授,你的要求不太清楚。I corrected you english based on my own understanding.  My research is so much different from yours.  It may not make sense to you.  Please do not feel insulted if I over do.  Hope this helps.  Good luck with your grant application. S2\ ;\?]^~  
]&P\|b1*g  
R ai 0 4  
Lepidium latifolium belongs to Brassicaceae, and is a relative to both Arabidopsis and Thellungiella halophila. It is a plant with broad spectrum stress tolerancse.  It has higher salt tolerant than llungiella salsuginea, and resistant to many other traumatic environments, such as chilliness and drought.  In Northern America, it is classified as a flood-tolerant and invasive species, nevertheless, it is widely distributed in salty and dehydrated soils in China.  The mechanism of salt resistance of llungiella salsuginea has been extensively studied.  Current understandings believe it is related to the constitution of many stress-related genes.  The molecular mechanism of salt and other stress resistance of T. halophila is generally considered relating to a set of constitutively expressed REDOX genes, such as SOD, POD,and P5CS etc.  The roots of Lepidium latifolium can differentiate  Na+ and K+ more efficiently than that of Arabidopsis.  The stress profile of L. latifolium is very similar to T. halophila, however, the underlying molecular mechanism of L. latifolium is still unknown.   Is there any common pathways/mechanisms shared by these high stress resistance plants? And if this common pathway/mechanism does exist, is it relevant to other plants without morphological and structural spcieation/differentiation? Answering these questions  are of significant importance in understanding universal stress tolerance. Based on our current knowledge of salt resistance physiology and Arabidopsis and Thellungiella genome and transcriptsome, we propose to invest the comparative transcriptsome and genome of L. latifolium.  Specifically, we will clone some stress tolerant genes. This may genetically improve salt and other stress tolerance of crops in Brassicaeae.
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伍胥之 威望 +10 2011-03-16 公报私恩啊!FB!
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地下室  发表于: 2011-03-16   
非常感谢!
杺栫杣杊椌柮栬,䒴蓉艿芖。
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5楼  发表于: 2011-03-16   
V1.01 |LbAW /9a  
-@_v@]:  
Lepidium latifolium belongs to Brassicaceae, and is a relative to both Arabidopsis and Thellungiella halophila. It is a plant with broad spectrum for stress tolerancse. It is much more tolerant to salt and many other traumatic environments such as cold and drought than that of  T. halophila. In Northern America, it is classified as a flood-tolerant and invasive species, nevertheless, it is widely distributed in salty and dehydrated soils in China. The mechanism of salt resistance of T. halophila has been extensively studied. Current understandings believe that it is closely related to a set of constitutively expressed REDOX genes, such as SOD, POD,and P5CS etc.  The roots of T. halophila can differentiate Na+ and K+ more efficiently than that of Arabidopsis.  The stress profile of L. latifolium is very similar to that of T. halophila, however, the underlying molecular mechanism of L. latifolium is still unknown.   Is there any common mechanisms shared by these high stress resistant plants?  And if this common mechanism does exist, is it relevant to other plants without morphological and structural specification? Answering these questions are of significant importance in understanding universal stress tolerance. Based on our current knowledge of salt resistance physiology,  we propose to carry out the comparative transcriptsome and genome of L. latifolium with Arabidopsis and Thellungiella.  Specifically, we will clone some stress tolerant genes. This may help in genetically improve salt and other stress tolerance of crops in Brassicaeae.
杺栫杣杊椌柮栬,䒴蓉艿芖。
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6楼  发表于: 2011-03-16   
spectrum用得特别精彩。
杺栫杣杊椌柮栬,䒴蓉艿芖。
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7楼  发表于: 2011-03-16   
明天早晨要打印出来,带到BJ去。
杺栫杣杊椌柮栬,䒴蓉艿芖。
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8楼  发表于: 2011-03-16   
Some small changes.
Lepidium latifolium belongs to Brassicaceae, and is a relative to both Arabidopsis and Thellungiella halophila. It is a plant with broad spectrum for stress tolerancse. It is much more tolerant to salt and many other traumatic environments such as cold and drought than that of  T. halophila. In Northern America, it is classified as a flood-tolerant and invasive species, nevertheless, it is widely distributed in salty and dehydrated soils in China. The mechanism of salt resistance of T. halophila has been extensively studied. Current understandings believe that it is closely related to a set of constitutively expressed REDOX genes, such as SOD, POD,and P5CS etc.  The roots of T. halophila can differentiate Na+ and K+ more efficiently than that of Arabidopsis.  The stress profile of L. latifolium is very similar to that of T. halophila, however, the underlying molecular mechanism of L. latifolium is still unknown.   Is there any common mechanisms shared by these high stress resistant plants?  And if this common mechanism does exist, is it universally applicable to other plants without morphological and structural specification? Answering these questions are of significant importance in understanding universal stress tolerance. Based on our current knowledge of salt resistance physiology,  we propose to carry out the comparative transcriptsome and genome analysis of L. latifolium with Arabidopsis and Thellungiella.  Specifically, we will clone some stress tolerant genes and study their expression and regulation. The findings from this study may significantly improve our understandings on how to  genetically improve salt and other stress tolerance of crops in Brassicaeae.
[ 此帖被suehan234在03-16-2011 19:56重新编辑 ]
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9楼  发表于: 2011-03-16   
Good luck.
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10楼  发表于: 2011-03-16   
拿到研究基金,分点钱给我:)
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11楼  发表于: 2011-03-16   
引用
引用第10楼suehan234于03-16-2011 13:27发表的  : m:?"|. ]  
拿到研究基金,分点钱给我:) m]AT-]*f  
[DM0'4  
v}Ju2}IK  
你居然知道Thellungiella salsuginea跟我用的T. halophila是同一个东东啊!显然也是圈内人啊!可惜了,早该写完让你看看全文给提点建议就好了。
杺栫杣杊椌柮栬,䒴蓉艿芖。
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12楼  发表于: 2011-03-16   
8hr已过~ I!S Eb  
ciS,  
SUEHAN234高手哇~ WrGnLE kiV  
@ps(3~?7  
膜拜呀
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13楼  发表于: 2011-03-23   
伍sri,非常对不住啊,俺咋就错过你这么重要的内容了,看时间已经过去一周了,你要求的8小时也已经过去了,实在不好意思,我这个外行没能帮上你内行的忙。 |vI`u[P  
看了一下suehan234帮你修改的内容,英语很专业,关键是你那学科她翻的专业,很多词儿我都没见过,说实话有点隔行如隔山,很是钦佩她。我也只是在英语的问法上能略略的帮上你点,专有名词还是拿捏不准,有些愧疚。 ?;ok9Y  
但无论如何才看到此贴,有点责怪自己,点着我大名呢,居然眼大漏神,自己竟然这么粗心,还请伍兄多见谅! CF '&Yo  
不过还是要求一下,要是得了啥奖,一定要分享一下的。
[ 此帖被清水在03-23-2011 20:52重新编辑 ]
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14楼  发表于: 2011-03-23   
太棒了,suehan234,水平这么高,伍色真有办法,学了一招,太需要这方面的帮助了。
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